Nucleotides – MCAT Biochemistry | MedSchoolCoach


MCAT Biochemistry - Chapter 4 - Section 1.1 - Nucleotides and Nucleic Acids - Nucleotides
Play Video about Nucleotides and Nucleic Acids Nucleotide Structure and Function

Sample MCAT Question - Nucleotides

Which of the following statements is correct regarding nucleotides and nucleosides?

a) Nucleotides consist only of a five-carbon sugar and a phosphate group

b) Nucleosides are an essential source of energy for metabolic processes

c) Nucleotides are the building blocks of RNA and DNA

d) Nucleosides are made up of nucleotides plus a phosphate group

C is correct. Nucleotides are the building blocks of RNA and DNA.

RNA is composed of nucleotides containing a ribose sugar, while DNA is made from those containing a deoxyribose sugar. Choice A is incorrect because nucleotides are made from a five-carbon sugar (ribose or deoxyribose), a phosphate group, and a nitrogenous base. Choice B is incorrect because it is nucleotides, not nucleosides, that constitute a source of energy for metabolic processes. Choice D is incorrect because it reverse the relationship between nucleosides and nucleotides. Nucelotides are made from nucleosides plus one or more phosphate groups, not the other way around.

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Nucleotide Structure and Function

Nucleotides are small biomolecules that are essential for all life. Three distinct components make up nucleotides: a 5-carbon sugar (ribose or deoxyribose), a nitrogenous base, and a phosphate group. This post discusses nucleotide structure, the difference between nucleotides vs nucleosides, and the role nucleotides play in living cells.


Sugars in Nucleotides (Ribose or Deoxyribose)

The 5-carbon sugar that makes up a nucleotide may be either a ribose or a deoxyribose. The difference between these two sugars is in the presence or absence of an oxygen atom at the 2’ carbon position. As you can see from Figure 1, a ribose sugar has a hydroxyl group at both the 2’ and 3’ carbon positions, while the deoxyribose has a hydroxyl group at the 3′ carbon only (hence the name “deoxyribose”).

Ribose vs Deoxyribose - MCAT Biochemistry
Figure 1. Ribose vs Deoxyribose

Nitrogenous Bases in Nucleotides

There are two types of nitrogenous bases found in nucleotides: purines and pyrimidines. Purines have two fused rings and include adenine and guanine (Figure 2). Pyrimidines, on the other hand, have one ring and include cytosine, thymine, and uracil (Figure 3).

Purines (Nitrogenous Bases) - MCAT Biochemistry
Figure 2. Purines (Nitrogenous Bases)
Pyrimidines (Nitrogenous Bases) - MCAT Biochemistry
Figure 3. Pyrimidines (Nitrogenous Bases)

Nucleotides vs Nucleosides

The combination of a 5′-carbon sugar (either ribose or deoxyribose) and a nitrogenous base (either a purine or a pyridine) is known as a nucleoside. Though the terms nucleoside and nucleotide sound similar, they are, in fact, distinct from one another. As mentioned earlier, a nucleotide consists of a 5-carbon sugar, a nitrogenous base, and a phosphate group, while a nucleoside, as we just mentioned, consists only the latter two components: a 5-carbon sugar and a nitrogenous base. In a sense, then, a nucleotide is a phosphorylated nucleoside, and a nucleoside is a dephosphorylated nucleotide.


To get a better sense of this distinction, look at Figures 4 and 5 below. Figure 4 shows a nucleoside called adenosine, which is made from ribose (a 5-carbon sugar) and adenine (a nitrogenous base). Figure 5, on the other hand, shows a nucleotide made from adenosine called adenosine monophosphate or AMP. As you can see, AMP has the same nucleoside structure as adenosine with the addition of a phosphate group.

Adenosine (a Nucleoside) - MCAT Biochemistry
Figure 4. Adenosine (a Nucleoside)
Adenosine Monophosphate (a Nucleotide) - MCAT Biochemistry
Figure 5. Adenosine Monophosphate (a Nucleotide)

It’s worth noting that nucleotides may have more than one phosphate a group. A famous example, and one related to AMP, is adenosine triphosphate, or ATP, which is the universal energy carrier found in all life on Earth. As you can see from Figure 6, ATP is identical to AMP, except that it has three phosphate groups rather than one.

Adenosine Triphosphate - MCAT Biochemistry
Figure 6. Adenosine Triphosphate

Nucleotide Biochemical Function

Nucleotides play several crucial roles in living cells:


– They are the building blocks of nucleic acids like DNA and RNA. Nucleotides made from deoxyribose combine to form DNA, while those made from ribose form RNA. As discussed in another post, DNA has an antiparallel, double-helical structure


– They serve as energy storage molecules. The most famous example, of course, is ATP, but other nucleotides like guanosine triphosphate (GTP) and uridine triphosphate (UTP) also play important roles in energy storage and transfer.


– They play a crucial role in cell signaling pathways. For instance, the nucleotide cyclic AMP (cAMP) mediates the intracellular effect of hormone binding at cell surface receptors.

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